Fig. 6

Reconstitution of Mfn2 alleviated the PAH phenotype in RC group. a, Schematic representation of the experimental procedures. b, Representative images of α-smooth muscle actin staining. Scale bar = 20 μm. c, Mean pulmonary arterial pressure of the rats (n = 8 or 4 per group). d, Medial wall thickness of pulmonary arteries in small (20–100 μm in diameter) pulmonary vessels. e, Right ventricular hypertrophy index (RV/ (LV + S)) of the rats (n = 8 or 4 per group). RC + Ad-Ev, RC group rats injected with control adenovirus; RC + Ad-Mfn2, RC group rats injected with recombinant adenovirus encoding Mfn2; mPAP, mean pulmonary arterial pressure; PA, pulmonary artery; RV, right ventricular hypertrophy index; LV, left ventricular; S, septum. All data throughout the figures represent the mean ± SEM and analyzed by one-way analysis of variance (ANOVA) and Newman-keuls post-hoc test. * p < 0.05 vs. Con + Ad-EV, ** p < 0.01 vs. Con + Ad-EV, # p < 0.05 vs. RC + Ad-EV. ## p < 0.01 vs. RC + Ad-EV