Fig. 4

Reconstitution of Mfn2 increased the fusion and mitochondrial functions of PASMCs in the RC group. a, Representative blot images of Mfn2 in rat lung tissues. b, Representative blot images of Mfn2 in rat PASMCs. c, Quantitative analysis of Mfn2 protein expression (n = 6–10 per group). d, Quantitative analysis of mitochondrial dynamics gene expression in lung tissues (n = 7 per group). e, Quantitative analysis of Mfn2 expression in rat PASMCs (n = 6 per group). f, Representative confocal microscope images showing mitochondrial morphology stained by MitoTracker Red. g, Representative images of mitochondrial membrane potential, scale bar = 50 μm. h, Quantitative analysis of mitochondrial membrane potential (n = 6 per group). i, Quantitative analysis of mitochondrial permeability transition pore in PASMCs (n = 4–7 per group). Ad-EV, control adenovirus; Ad-Mfn2, recombinant adenovirus encoding Mfn2, MPTP, mitochondrial permeability transition pore. All data throughout the figures represent the mean ± SEM. Figures c-e were analyzed by Student’s two-tailed t-test and Figures h-i were analyzed by one-way analysis of variance (ANOVA) and Newman-keuls post-hoc test. * p < 0.05, ** p < 0.01 compared to CC group, $ p < 0.05 vs. Con + Ad-EV, $$ p < 0.01 vs. Con + Ad-EV, # p < 0.05 vs. RC + Ad-EV. ### p < 0.001 vs. RC + Ad-EV