Fig. 3

RC group rats exhibited dysregulated PASMC functions and mitochondrial protein expressions. a, Representative images of migration of pulmonary arterial smooth muscle cells, scale bar = 50 μm. b, Statistics of the PASMCs migration (n = 6–8 per group). c, Statistics of the PASMCs proliferation (n = 6–7 per group). d, Representative images of PASMC mitochondrial oxidative stress staining, scale bar = 20 μm. e, Statistics of fluorescence intensity (n = 5 per group). f, Statistics of relative fluorescence unit of MPTP (n = 7 per group). g, Representative blot images of AMPK and pAMPK isolated from lung tissues (n = 6 per group). h, analysis of expression of AMPK and pAMPK in lung tissues. i, Representative blot images of mitochondrial specific proteins VDAC and PGC1α isolated from lung tissues (n = 6 per group). j, analysis of expression of VDAC and PGC1α in lung tissues. MPTP, mitochondrial permeability open pores. All data throughout the figures represent the mean ± SEM and analyzed by Student’s two-tailed t-test. * p < 0.05, ** p < 0.01, *** p < 0.001 compared to CC group