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Fig. 5 | Respiratory Research

Fig. 5

From: Ubiquitination of angiotensin-converting enzyme 2 contributes to the development of pulmonary arterial hypertension mediated by neural precursor cell–expressed developmentally down-regulated gene 4-Like

Fig. 5

Overexpression(OE) of ACE2 can partially reverse the inhibition of cell apoptosis by NEDD4L. PASMCs in the two groups were treated with NEDD4L or NEDD4L + ACE2. The expression of NEDD4L and ACE2 in the three groups was examined (A-C). Then, PAMSCs were aspirated at 0, 24, 48, and 72 h after transfection, and their cell activity was examined through a CCK-8 assay (D). Cell apoptosis was observed by flow cytometry (E-H). Finally, IF (I, K) and EdU (J, L) staining were performed to observe the expression of ACE2 and cell proliferation in PASMCs. n = 6, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. NEDD4L, neural precursor cell–expressed developmentally downregulated gene 4-like; ACE2, angiotensin-converting enzyme 2; PAH, pulmonary arterial hypertension; GAPDH, glyceraldehyde-3-phosphate dehydrogenase

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Respiratory Research

ISSN: 1465-993X

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