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Fig. 2 | Respiratory Research

Fig. 2

From: Ubiquitination of angiotensin-converting enzyme 2 contributes to the development of pulmonary arterial hypertension mediated by neural precursor cell–expressed developmentally down-regulated gene 4-Like

Fig. 2

NEDD4L may be the E3 ubiquitin ligase of ACE2 and inhibit the expression of ACE2 in PAH. In the network view, a node is positioned in the center of the canvas, showing the putative substrate ACE2, which is surrounded by nodes revealing predicted E3 ligases. The node colors and characteristics denote the E3 ligase type, with the edge width and node size representing the confidence score. In the confidence mode (A), both edge width and node size positively correlate with the UbiBrowser score. The top 1 to 6 genes were selected for qPCR to observe the effect of hypoxia and MG-132 on mRNA expression (B-H), and then, NEDD4L and MDM2 were selected for western blot analysis to observe the effect of hypoxia and MG-132 on protein expression. GAPDH was used as an internal reference (I-J). n = 6, **P < 0.01. NEDD4L, neural precursor cell–expressed developmentally downregulated gene 4-like; ACE2, angiotensin-converting enzyme 2; PAH, pulmonary arterial hypertension; qPCR, quantitative PCR; MDM2, mouse double minute 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase

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Respiratory Research

ISSN: 1465-993X

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