Fig. 6

PM@TP/NPs relieved lung inflammation in asthmatic mice. (A-B) Infiltrated cells in the lung with normal mice and HDM-induced asthmatic mice (n = 5 per group) were isolated and total cell counts, eosinophils, neutrophils, macrophages and lymphocyte cells in the lung were calculated by flow cytometry. (C) Representative lung inflammatory cells were prepared by flow cytometry analysis. (D) Representative micrographs of lung tissue cross-sections from mice with normal mice and HDM-induced asthma were stained by H&E (n = 3). Bars: 100 μm. (E) The mRNA expression of pro-inflammatory cytokines from normal mice and HDM-induced asthmatic mice (n = 5 per group) with or without treatment was examined by RT-qPCR. (F-G) Th-producing cells in the lungs from normal mice and HDM-induced asthmatic mice (n = 5 per group) were isolated and followed by flow cytometry analysis. *p < 0.05; **p < 0.01