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Fig. 6 | Respiratory Research

Fig. 6

From: Mendelian randomization study on causal association of TEF and circadian rhythm with pulmonary arterial hypertension

Fig. 6

Functional validation of TEF in PAH. (A) Representative immunofluorescence staining of α-SMA (green) and TEF (red) in PASMCs from the control and PAH groups (scale bar: 100 μm). (B) Efficiency of TEF silencing and overexpression in PASMCs. (C) Representative immunofluorescence staining of CFDA-SE (green) and DAPI (blue) in PASMCs, with or without treatment with PDGF-BB. CFDA-SE, Cell proliferation and tracking dye. (D) Representative images of PASMCs migration in different groupsassessed by Transwell. (E) Expression levels of IL6 and IL-1β in different groups assessed by Specific ELISA kits

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