Fig. 7

Establishment of MCT induced PH mouse model. (A) RVSP and (B) RV/(LV + S) were assessed in MCT-induced PH rats or control (n = 8/group). (C) Representative images of H&E and EVG staining of lung tissues in two group. Black asterisk indicates lumen of pulmonary artery. Scale bar = 30 mm. (D) Quantification of media thickness of small pulmonary arterioles (E) The mRNA expression level of TFRC relative to GAPDH were examined in lung tissues from MCT-induced PH mice or control. (n = 5/group). (F) Representative Western blots and quantification of TFRC and GAPDH in the lungs of MCT- induced rats and controls. RVSP = right ventricular systematic pressure; RV/(LV + S) = right ventricular/(left ventricular + septum). Data represent mean ± SEM. *P < 0.05; **P < 0.01 compared to control, as analyzed by unpaired t test or Mann-Whitney test as appropriate