Fig. 5

The association between PGK1 and NLRP3 exhibited an increased affinity after being stimulated by LPS A-B: Co-IP assays were performed to detect the interaction between NLRP3 and PGK1 using the lysate of BMDMs. C-D: Co-IP assays were performed to detect the interaction between NLRP3 and PGK1 using the lysate of mice’s lung tissue. E-F: Molecular binding between NLRP3 and PGK1 protein is graphically displayed using the HDOCK Server. The ideal predicted docking position of NLRP3(blue ribbon) on the domain where ADP binds to PGK1(pink ribbon)(E). The red circle indicates the specific locations on the amino acid sites where the modification of NLRP3 may occur(F). The data shown are the means ± SEM obtained from three separate biological replicates. * P < 0.05 by unpaired t test