Fig. 4

An interaction exists between PGK1 and NLRP3 in BMDMs under the stimulation of LPS A: BMDMs were exposed to LPS for a duration of 24 h in order to model the features of inflammatory macrophages. The intersection analysis of the correlation between NLRP3 IP-MS and the aerobic glycolysis gene set (M39816) from the msigdb database. Identification of PGK1 as a potential NLRP3 interactor in LPS-stimulated BMDMs by mass spectrum primed with LPS. B: PGK1 expression in different cells of lung tissue from The Human Protein Atlas (HPA) database. C:Co-IP analysis of endogenous association between PGK1 and NLRP3 in LPS-stimulated BMDMs. D: Immunofluorescence staining for the colocalization of NLRP3(Green) and Cleaved-caspase1(Red) (scale bar 100 μm). E-G: Western blotting of NLRP3 and PGK1 protein levels at different times. The data shown are the means ± SEM obtained from three separate biological replicates. * P < 0.05, ** P < 0.01, ns, not significant by one-way ANOVA