Fig. 3

Elevated presence of dysmorphic cells with spindle phenotype in BCs from donor LUSC patients. (A) Median and quartiles showing the percentage of spindled cells in all four groups as quantified by two independent double blinded individuals. Data from passage 2 never-smoker (Never), smoker, lung adenocarcinoma (LUAD), and squamous cell carcinoma (LUSC) cells are shown (n ≥ 3; *P < 0.05 by ANOVA). (B) Immunofluorescent micrographs from never smoker and lung squamous cell carcinoma (LUSC) patient cultured cells, labeled from left to right with; Top: DAPI nuclear counterstain (blue), vimentin (VIM; green) and N-cadherin (CDH2; red), and merge. Below: BCs from separate never and LUSC patients labeled from left to right with; DAPI (blue), cyclin D1 (CCND1; green) and cytokeratin 14 (KRT14; red), and merge. Labeling in cells from donor smokers and LUAD did not differ significantly from never smokers