Fig. 5

Low expression of CNOT4 induced EEF2-mediated senescence of AECII in IR. (A), CNOT4 expression level on AECII Cells with 0, 4, 6, 8, 10 Gy after 48 h. (B), the quantification of (A). (C), CNOT4 expression level on AECII Cells at the indicated time points following exposing to 10 Gy IR. (D), the quantification of (C). (E), CNOT4 expression level on AECII Cells after over-expression TNKS1BP1 with 4 μg plasmid transfected. (F), the quantification of (E). (G), CNOT4 expression level on ACEII Cells after inhibition of TNKS1BP1. (H), the quantification of (G). (I), CNOT4 expression level on ACEII Cells after inhibition of TNKS1BP1 simultaneously under IR. (J), the quantification of (I). (K), EEF2, P53, and P21 expression level on ACEII Cells after inhibition of CNOT4. (L), the quantification of (K). (M), co-IP assay analyzing the interaction of TNKS1BP1 and CNOT4 after radiation. Data are presented as mean ± SD. For statistical analysis, B, D, F, H and L were conducted by unpaired Student’s t test; J was conducted by One-way ANOVA, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 mean the difference statistical significance