Fig. 4

Over-expression TNKS1BP1 promoted EEF2 degradation. (A), EEF2 expression level on AECII Cells after inhibition TNKS1BP1 and treated with MG132. (B), EEF2 expression level on AECII Cells after inhibition TNKS1BP1 and treated with Cycloheximide (CHX). (C) co-IP assay analyzing the interaction of TNKS1BP1 and CNOT4. (D), co-IP assay analyzing the interaction of EEF2 and CNOT4, and the ubiquitin degradation of EEF2 after over-expression TNKS1BP1