Fig. 3

Under IR, high TNKS1BP1 expression promoted AECII senescence through up-regulation of EEF2. (A), the interaction of TNKS1BP1 and EEF2 on AECII Cells identified by co-IP. (B), EEF2 expression level on AECII Cells with 0, 4, 6, 8, 10 Gy after 48 h. (C), the quantification of (B). (D), EEF2 expression level on AECII Cells at the indicated time points following exposing to 10 Gy IR. (E), the quantification of (D). (F), EEF2 expression level on AECII Cells after over-expression TNKS1BP1 with 4 μg plasmid transfected. (G), the quantification of (F). (H), EEF2 expression level on ACEII Cells after inhibition of TNKS1BP1 simultaneously under IR. (I), the quantification of (H). (J), P53 expression level on ACEII Cells after inhibition of EEF2. (K), the quantification of (J). (L), P53 and P21 expression level on ACEII Cells after inhibition of EEF2 simultaneously under IR. (M), the quantification of (L). Data are presented as mean ± SD. For statistical analysis, C, E and G were conducted by unpaired Student’s t test; I, K and M were conducted by One-way ANOVA, ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 mean the difference statistical significance