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Fig. 1 | Respiratory Research

Fig. 1

From: Precision cut lung slices: an integrated ex vivo model for studying lung physiology, pharmacology, disease pathogenesis and drug discovery

Fig. 1

Generation of human lung slices. The lobes of the lungs from either healthy or diseased donors were inflated with a low melting point agarose, the agarose was allowed to solidify in the lungs, and the lung tissue was sectioned. A tissue punch or coring tool was used to generate columns of tissue containing airways, which are fed into a Precisionary Instruments Vibratome© to generate ~350 µm thick slices. Airways in these slices were identified, and the slices were weighed by platinum weights with nylon threads to assess changes in contraction and relaxation of the airways

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Respiratory Research

ISSN: 1465-993X

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