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Table 1 Sequences of quantitative RT-PCR primers

From: Statin administration or blocking PCSK9 alleviates airway hyperresponsiveness and lung fibrosis in high-fat diet-induced obese mice

Genes

Forward primers sequence (5’→3’)

Reverse primers sequence (5’→3’)

Collagen 1_Ms

TGGGATTCCCTGGACCTAA

GCTCCAGCTTCTCCATCTTT

Collagen 3_Ms

ATCTGAGGGCTCGCCCGGT

CAATGGCAGCACCGCCACCA

Fibronectin_Ms

TCAGAAGAGTGAGCCCCTGA

GGAAGGGTAACCAGTTGGGG

NLRP3_Ms

GCTGCTGAAGATGACGAGTG

TTTCTCGGGCGGGTAATCTT

Caspase-1_Ms

TCATTTCCGCGGTTGAATCC

CCAACAGGGCGTGAATACAG

ASC_Ms

ATGCCAACCAAAGCCAGAAG

CCTTGGGGTTGGAGAGATGA

IL-1β_Ms

GGCTCATCTGGGATCCTCTC

TCATCTTTTGGGGTCCGTCA

β-actin_Ms

CGCCACCAGTTCGCCATGGA

TACAGCCCGGGGAGCATCGT

GAPDH_Hm

CACATCGCTCAGACACCATG

TGACGGTGCCATGGAATTTG

NLRP3_Hm

TCTCATGCTGCCTGTTCTCA

CAAGGAGATGTCGAAGCAGC

Caspase-1_Hm

CCGAGCTTTGATTGACTCCG

TTCTGAGCCTGAGGATGTGG

IL-1β_Hm

GGAGAATGACCTGAGCACCT

GGAGGTGGAGAGCTTTCAGT

  1. (Ms: mouse, Hm: human)
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Respiratory Research

ISSN: 1465-993X

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