Fig. 6

ISM1 regulates adiponectin expression in the lungs. (A) Relative adiponectin protein expression in lung lysates (n = 5). Data are presented as the mean ± SD. *P < 0.05, using Student’s t test. (B) Adiponectin level in the BALF of HDM-challenged mice compared with the saline control measured with ELISA (n = 5). Data are presented as the mean ± SD. ****P < 0.0001, using two-way ANOVA. (C) Immunofluorescence images showing colocalization between adiponectin and SP-C in WT lungs. Bars = 20 μm. (D) Adiponectin expression in conditioned media from A549 cells treated with different doses of rISM1. (E-F) PPARγ protein expression and quantification in A549 cell lysates post-treated with 200 nM of rISM1 measured at different time points. (G-H) Adiponectin expression and quantification in A549 conditioned media under 200 nM rISM1 with or without GRP78 antibody neutralization. Data are presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, using one-way ANOVA. SP-C, surfactant protein C; PPARγ, peroxisome proliferator-activated receptor gamma; GRP78, glucose-regulating protein 78