Fig. 6

SNHG16 regulated expression of Notch2 by targeting miR-455-3p. a Notch2 mRNA wide-type (Notch2 WT) and the mutated-type (Notch2 MUT) in the miR-455-3p binding sites were shown. b Luciferase activity of HEK293 cells co-transfected with miR-455-3p mimics or NC mimics and luciferase reporters containing Notch2 3′-UTR WT or Notch2 3′-UTR MUT transcript were determined by dual-luciferase reporter assays. c The expression level of protein Notch2 was detected in miR-455-3p mimic, inhibitor and their control groups (control, NC-inh) by western blot. d The expression level of protein Notch2 were analyzed by western blot in TGF-β1 treated lung fibroblast cells which co-transfected with sh-SNHG16 or its control (sh-NC) and miR-455-3p inhibitor or its control (NC-inh). Data were expressed as mean ± SD. **P < 0.01 vs control; ^##P < 0.01 vs NC-inh or TGF-β1 + Ad sh-NC; ^$$P < 0.01 vs TGF-β1 + Ad sh-SNHG16, represent statistically difference