Fig. 2

The presence of cilia at the surface of the cells confirms that H292 cells were grown at the air–liquid interface (ALI). a Characterization of the ALI cell culture model. H&E staining reveals the multiple cell layers on the apical surface following 1 day of exposure to either air or butter-flavored e-cig aerosol. b Scanning electron microscopy images of representative H292 cells exposed to either air or butter-flavored e-cig aerosols for 1 day at the ALI, with a higher magnification of the cilia present at the surface of the cells. Images showed are representative for each exposure group. For air-exposed cells: a total of 38 SEM images were taken; for butter-flavored e-cig aerosol-exposed cells: a total of 53 SEM images were taken